Selected Publications
Wang S, Speck NA (1992). Purification of core-binding factor, a protein that binds the conserved core site in murine leukemia virus enhancers. Mol Cell Biol. 12 (1): 89-102.
Wang S, Wang Q, Crute BE, Melnikova IN, Keller SR, Speck NA (1993). Cloning and characterization of subunits of the T cell receptor and murine leukemia virus enhancer core-binding factor. Mol Cell Biol. 13 (6): 3324-3339.
Wang Q, Stacy T, Binder M, Marín-Padilla M, Sharpe AH, Speck NA (1996). Disruption of the CBFα2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. Proc Natl Acad Sci USA. 93 (8): 3444-3449.
Wang Q, Stacy T, Miller JD, Lewis AF, Gu TL, Huang X, Bushweller JH, Bories JC, Alt FW, Ryan G, Liu PP, Wynshaw-Boris A, Binder M, Marín-Padilla M, Sharpe AH, Speck NA (1996). The CBFβ subunit is essential for CBFα2 (AML1) function in vivo. Cell 87 (4): 697-708.
North T, Gu TL, Stacy T, Wang Q, Howard L, Binder M, Marín-Padilla M, Speck NA (1999). CBFα2 is required for the formation of intra-aortic hematopoietic clusters. Development 126 (11): 2563-2575.
Bravo J, Li Z, Speck NA, Warren AJ (2001). The leukemia-associated AML1 (Runx1)-CBFβ complex functions as a DNA-induced molecular clamp. Nat Struct Biol. 8 (4): 371-378.
Speck NA, Gilliland DG (2002). Core-binding factors in hematopoiesis and leukemia. Nat Rev Cancer 2 (7): 502-513.
North TE, de Bruijn MF, Stacy T, Talebian L, Lind E, Robin C, Binder M, Dzierzak E, Speck NA (2002). Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. Immunity 16 (5): 661-972.
Lukasik S, Zhang L, Corpora T, Tomanicek S, Li Y, Kundu M, Hartman K, Liu PP, Laue TM, Biltonen RL, Speck NA, Bushweller JH (2002). Altered affinity of CBFβ-SMMHC for Runx1 explains its role in leukemogenesis. Nat Struct Biol. 9 (9): 674-679.
Miller, J, Horner A, Stacy T, Lowrey C, Lian JB, Stein G, Nuckolls GH, Speck NA (2002). The core-binding factor β subunit is required for bone formation and hematopoietic maturation. Nat Genet. 32 (4): 645-649.
Li Z, Yan J, Matheny CJ, Corpora T, Bravo J, Warren AJ, Bushweller JH, Speck NA (2003). Energetic contribution of residues in the Runx1 Runt domain to DNA binding. J Biol Chem. 278 (35), 33088-33096.
Zhang, L, Li Z, Yan J, Pradhan P, Corpora T, Cheney MD, Bravo J, Warren AJ, Bushweller JH, Speck NA (2003). Mutagenesis of the Runt domain defines two energetic hotspots for heterodimerization with the core binding factor β subunit. J. Biol Chem. 278 (35): 33097-33104
Yan J, Liu Y, Lukasik SM, Speck NA, Bushweller JH (2004). CBFβ allosterically regulates the Runx1 Runt domain via a dynamic conformational equilibrium. Nat Struct Mol Biol. 11 (9): 901-906.
Li Z, Chen MJ, Stacy T, and Speck NA (2006). Runx1 function in hematopoiesis is required in cells that express Tek. Blood 107 (1): 106-110.
Liu Y, Cheney MD, Gaudet JJ, Chruszcz M, Lukasik SM, Sugiyama D, Lary J, Cole J, Dauter Z, Minor W, Speck NA*, Bushweller JH* (2006). The tetramer structure of the Nervy homology two domain, NHR2, is critical for AML1/ETO's activity. Cancer Cell, 9 (4): 249-260 (*corresponding authors
Zeigler BM, Sugiyama D, Chen M, Guo Y, Downs KM*, and Speck NA* (2006). The allantois and chorion, isolated before circulation or chorio-allantoic fusion, have hematopoietic potential. Development 133 (21): 4183-4192, 2006. (*corresponding authors).
Talebian L, Li Z, Guo Y, Gaudet JJ, Speck ME, Sugiyama D, Kaur P, Pear WS, Maillard I*, and Speck NA* (2007). T lymphoid, megakaryocyte, and granulocyte development are sensitive to decreases in CBFβ dosage. Blood (Plenary Paper) 109 (1): 11-21. (*corresponding authors)
Matheny CJ, Speck ME, Cushing P, Zhou Y, Corpora T, Regan M, Newman M, Roudaia L, Speck CL, Gu TL, Griffey SM, Bushweller JH*, and Speck NA* (2007). Mutations in the Runt domain found in hematopoietic disorders and cleidocranial dysplasia create nonfunctional, dominant negative, and hypomorphic alleles. EMBO J. 26 (4): 1163-1175 (*corresponding authors)
Liu Y, Chen W, Gaudet J, Cheney MD, Roudaia L, Cierpicki T, Klet RC, Hartman K, Laue TM, Speck NA*, Bushweller JH* (2007). Structural basis for recognition of SMRT/N-CoR by the MYND domain and its contribution to AML1/ETO's activity. Cancer Cell 11 (6): 483-497 (*corresponding authors).
Gorczynski MJ, Grembecka Y, Zhou Y, Kong Y, Roudaia L, Douvas MG, Newman M, Baber G, Bielnicka IZ, Corpora T, Shi J, Sridharan M, Lilien R, Donald BR, Speck NA, Brown MA, Bushweller JH. (2007) Development of an allosteric inhibitor of the protein-protein interaction between the leukemia-associated proteins RUNX1 and CBFβ. Chem Biol. in press.