Expertise
Scott Gleim (Hwa lab): protein purification, UV-Vis spectroscopy
Eric Arehart (Hwa lab): competition/saturation binding, cAMP assay
Ryan Horvath (DeLeo Lab): Transwell migration assay, immunocyto(histo)chemistry
Courtney Kozul (Hamilton Lab): Microarray Analysis
Shohreh Farzan (Robbins Lab): Ultracentrifugation and subcellular fractionation
Samar Singh (Robbins Lab): FPLC
Xi (Hill) Liu (Dmitrovsky Lab): transgenic mice for lung cancer, primary epithelia (lung cancer) cell culture, microRNA
Athena Nomikos (Hamilton Lab): Bioanalyzer, real time PCR
Kristina Fetalvero (Martin lab): immunoprecipitation, Nucleofector transfection, PCR
Min Ding (Martin Lab): Immunoprecipitation, Nucleofector for siRNA and plasmid transfections
DeLeo Lab Expertise:
Rodent Spinal Cord Surgery
Rodent Spinal Cord/Brain Tissue perfusion and Harvest
Immunohistochemistry: Free Floating Tissue, SuperFrost Slide, DAB and immunofluorescence
Immunocytochemistry: rodent cell cultures
Flow Cytometry: rodent cell culture, ex vivo spinal cord cells
Western Blotting of rodent proteins
ELISA of rodent cytokines
Transwell migration assay: rodent primary microglial and immortilized cultures
Animal Behavorial Testing: Rodent-hyperalgesia, alloydia, righting, writhing, step placement
rodent spinal cord, brain, spleen tissue cutting using a Cryostat