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Robert A. Maue, Ph.D.
Title(s):
Professor of Physiology and of Biochemistry
Department(s):
Biochemistry
Physiology
Education:
U. California - San Diego, PHD 1985
Programs:
Molecular and Cellular Biology Graduate Programs
Neuroscience Center at Dartmouth
Program in Experimental and Molecular Medicine
Websites:
http://dms.dartmouth.edu/ncd/
Contact Information:
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Dartmouth Medical School HB 7701 Hanover NH 03755 |
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Office: Remsen 328 Phone: 603-650-1311/ 650-1119 (l Fax: 650-1128 Email: robert.maue@dartmouth.edu |
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Selected Publications: |
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Paul CA, Boegle AK, Maue RA. Before the loss: neuronal dysfunction in Niemann-Pick Type C disease. Biochim Biophys Acta. 2004 Oct 11;1685(1-3):63-76. Review. (view details on MedLine) Paul CA, Reid PC, Boegle AK, Karten B, Zhang M, Jiang ZG, Franz D, Lin L, Chang TY, Vance JE, Blanchette-Mackie J, Maue RA. Adenovirus expressing an NPC1-GFP fusion gene corrects neuronal and nonneuronal defects associated with Niemann pick type C disease. J Neurosci Res. 2005 Sep 1;81(5):706-19. (view details on MedLine) Fry M, Boegle AK, Maue RA. Differentiated pattern of sodium channel expression in dissociated Purkinje neurons maintained in long-term culture. J Neurochem. 2007 May;101(3):737-48. (view details on MedLine) Maue RA. Understanding ion channel biology using epitope tags: progress, pitfalls, and promise. J Cell Physiol. 2007 Dec;213(3):618-25. Review. (view details on MedLine) |
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Professional Interests:
We are interested in understanding the mechanisms important for the development and differentiation of neurons in the brain. In particular, we are interested in the means by which a family of growth factors known as neurotrophins (NGF, BDNF, NT-3) exert their influence the growth and electrical activity of neurons, including their regulation of ion channel expression. Our recent efforts have focused on a class of neurons in the brain known as cerebellar Purkinje cells, and our interest in events associated with their development has also included the pathology and abnormal development of these neurons in neurological disorders such as Niemann Pick Type C (NPC) disease. NPC disease is a fatal genetic disorder associated with abnormal cellular cholesterol accumulation, and a hallmark of this disorder is the preferential loss of Purkinje cells. Given the recent appreciation of the importance of cholesterol in the brain and the similarities between NPC disease, Alzheimer’s disease, and other neurodegenerative disorders, understanding the effects of NPC disease on Purkinje cells has widespread implications.
In our experiments we have taken advantage of transgenic mice expressing ion channel genes, knockout mice lacking neurotrophin receptors, and novel mouse models of NPC disease that we’ve developed to analyze neurons in primary culture, in brain slices, and in vivo. This has included immunocytochemical and morphometric analyses, Western blotting and biochemical assays, single-cell, real time PCR analyses of gene expression, patch clamp recording of ion channels and electrical activity, virus-mediated expression of fluorescent proteins in vivo and in cultured neurons, and behavioral analyses of cerebellar function. Among future studies include behavioral and histological evaluation of potential treatments for NPC disease, and analysis of genes encoding ion channels, calcium binding proteins, and enyzmes involved in cholesterol metabolism in wild type and mutant Purkinje cells, using single-cell, real time PCR.
Courses Taught:
Instructor Medical Neuroscience (Medical student course) 1990-present
Instructor PEMM Core Course (Grad student course – PEMM Program) 2007-present
Director/Lecturer Adv Biomed Sci (Grad student course – PEMM Program) 2008-present
Instructor Molec Cell Biol (Grad student core course - MCB Program) 1996-2005
Instructor Receptor Pharm(Grad student course - Pharm Program) 1996-2006
Director/Instruct Cell / Molec Neuro (PBS 46 –upper div undergrad course) 2007-present
Director/Instruct Physiology (Bio 35 –upper division undergrad course) 2006
Director/Instruct Adv Neurobiology (Bio 74 –upper div undergrad course) 2006
Instructor Biochemistry (Bio 78 –upper division undergrad course) 1998-2005
Instructor Intro Cell Biology (Bio 15 –upper division undergrad course) 2003
Grant Information:
National Institutes of Health, National Niemann Pick Disease Foundation, Ara Parseghian Medical Research Foundation, Hereditary Disease Foundation, Epilepsy Foundation, Wellcome Trust
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