Images
The major cellular contributors of chronic pain states and their interface with the microvasculature, including neurons, microglia, astrocytes, perivascular microglia and T-leukocytes are illustrated. Potential drug targets to modulate glial-neuronal function include: (1) inhibiting microglial proliferation and migration, (2) enhancing astrocytic GLT-1 expression, (3) enhancing perivascular ED2/CD163 expression, and (4) interfering with specific neuronal-glial and/or neuronal-immune interactions.
Abcam rabbit anti-GFAP (ab7260) 1:5000
Alexa-488 goat anti-rabbit
Cortical rat pup prep.
Astrocyte culture
Abcam mouse anti-s100 (ab7260) 1:100
Alexa-555 goat anti-mouse
Cortical rat pup prep.
Astrocyte culture
ABcamS100
Abcam ab3561
Green=CB2 staining, Blue=DAPI staining
Click on the two images above to play video.
Many technicques are applied within the laboratory including:
Immunohistochemical Staining
Left panel: microglia - Right panel: astrocytes
Flow Cytometric Analysis
L-glu 10-cells stimulated with 10 uM L-glutamate with and without pre-treatment with minocycline
Fluorescence Microscopy
Spinal nerve transection tissue labeled with CB2, DAPI, a nuclear marker and GFAP, a marker for astrocytes