Publications
Research Articles:
Bue C.A., Barlowe C. (2009) Molecular dissection of Erv26p identifies separable cargo binding and coat protein sorting activities. J Biol Chem. Jul 1. [Epub ahead of print] PMID: 19574229.
Dancourt J, Barlowe C. (2009) Erv26p-dependent export of alkaline phosphatase from the ER requires lumenal domain recognition. Traffic 10, 1006-1018.
Lorente-Rodriguez A, Heidtman M, Barlowe C. (2009) Multicopy suppressor analysis of thermosensitive YIP1 alleles implicates GOT1 in transport from the ER. J Cell Sci. 122, 1540-1550.
Gitler A.D., Bevis B.J., Shorter J., Strathearn K.E., Hamamichi S., Su L.J., Caldwell K.A., Caldwell G.A., Rochet J.C., McCaffery J.M., Barlowe C., Lindquist S. (2008) The Parkinson's disease protein alpha-synuclein disrupts cellular Rab homeostasis. Proc. Natl. Acad. Sci. USA 105, 145-150.
Behni R, Barr F.A., Flanagan J.J., Barlowe C., Munro S. (2007) The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic. J. Cell Biol. 176, 255-261.
Bue C.A., Bentivoglio C.M., Barlowe C. (2006) Erv26p Directs Pro-Alkaline Phosphatase into ER-derived COPII Transport Vesicles. Mol. Biol. Cell. 17, 4780-4789.
Wilson, J.D., Liu, Y., Bentivoglio, C.M., Barlowe, C. (2006) Sel1p/Ubx2p participates in a distinct Cdc48p-dependent endoplasmic reticulum-associated degradation pathway. Traffic 7, 1213-1223.
Flanagan, J.J., Barlowe, C. (2006) Cysteine-disulfide crosslinking to monitor SNARE complex assembly during ER-golgi transport. J Biol Chem. 281, 2281-2288.
Miller, E.A., Liu, Y. Barlowe, C., Schekman, R. (2005) ER-Golgi transport defects are associated with mutations in the Sed5p-binding domain of the COPII coat subunit, Sec24p. Mol. Biol. Cell 16, 3719-3726.
Ballew, N., Liu, Y., Barlowe, C. (2005) A Rab requirement is not bypassed in SLY1-20 suppression. Mol. Biol. Cell 16, 1839-1849.
Heidtman, M., Chen, C.Z., Collins, R.N., Barlowe, C. (2005) Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the Golgi complex. Mol. Biol. Cell 16, 1673-1683.
Chen, C.Z., Calero, M., DeRegis, C.J., Heidtman, M., Barlowe, C., Collins, R.N. (2004) Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized Rab proteins and Rab-Guanine nucleotide dissociation inhibitor. Genetics 168, 1827-1841.
Otte, S. and Barlowe, C. (2004) Sorting signals can direct receptor-mediated export of soluble proteins into COPII vesicles. Nat. Cell Biol. 6, 1189-1194.
Breuza, L., Halbeisen, R, Jeno, P., Otte, S., Barlowe, C., Hong, W., Hauri, H.P. (2004) Proteomics of ERGIC membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46. J Biol Chem. 279, 47242-47253.
Liu, Y., Flanagan, J.J. and Barlowe, C. (2004) ER Export of Sec22p is Independent of SNARE Pairing. J Biol Chem. 279, 27225-27235.
Heidtman, M., Chen, C.Z., Collins, R.N. and Barlowe, C. (2003) A Role for Yip1p in COPII Vesicle Biogenesis. J. Cell Biol. 163, 57-69.
Orci, L., Ravazzola, M., Mack, G.J., Barlowe, C. and Otte, S. (2003) Mammalian Erv46 localizes to the endoplasmic reticulum-Golgi intermediate compartment and to cis-Golgi cisternae. Proc. Natl Acad. Sci. USA 100, 4586-4591.
Otte, S. and Barlowe, C. (2002) The Erv41p-Erv46p complex: Multiple export signals are required in trans for COPII-dependent transport from the ER. EMBO J. 21, 6095-6104.
Liu, Y., and Barlowe, C. (2002) Analysis of Sec22p in Endoplasmic Reticulum/Golgi Transport Reveals Cellular Redundancy in SNARE Protein Function. Mol. Biol. Cell 13, 3314-3324.
Powers, J. and Barlowe, C. (2002) Erv14p Directs a Transmembrane Secretory Protein into COPII Coated Transport Vesicles. Mol. Biol. Cell 13, 880-891.
Belden, W.J. and Barlowe, C. (2001) Role of Erv29p in collecting soluble secretory proteins into ER-derived transport vesicles. Science 294, 1528-1531.
Vashist, S., Kim, W., Belden, W.J., Spear, E.D., Barlowe C. and Ng, D.T. (2001) Distinct retrieval and retention mechanisms are required for the quality control of endoplasmic reticulum protein folding. J. Cell Biol. 155, 355-368.
Belden, W.J. and Barlowe, C. (2001) Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex. J. Biol. Chem. 276, 43040-43048.
Belden, W.J. and Barlowe C. (2001) Deletion of yeast p24 genes activates the unfolded protein response. Mol. Biol. Cell 12, 957-969.
Otte, S., Belden, W.J., Heidtman, M., Liu, J., Jensen, O.N. and Barlowe, C. (2001) Erv41p and Erv46p: New components of COPII vesicles involved in transport between the ER and Golgi complex. J. Cell Biol. 152, 503-517.
Brigance, W.T., Barlowe, C., Graham, T.R. (2000) Organization of the yeast Golgi complex into at least four functionally distinct compartments. Mol. Biol. Cell. 11:171-182.
Cao, X. and Barlowe, C. (2000) Asymmetric requirements for a Rab GTPase and SNARE proteins in fusion of COPII vesicles with acceptor membranes. J. Cell Biol. 149, 55-65.
VanRheenen, S.M., Cao, X, Sapperstein, S.K., Chiang, E.C., Lupashin, V.V., Barlowe, C. and Waters, M.G. (1999) Sec34p, a protein required for vesicle tethering to the yeast Golgi apparatus, is in a complex with Sec35p. J. Cell Biol.147, 729-742.
Conchon, S., Cao, X., Barlowe, C. and Pelham, H.R.B. (1999) Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex. EMBO J. 18, 3934-3946.
Powers, J. and Barlowe, C. (1998) Transport of Axl2p depends on Erv14p, an ER-vesicle protein related to the Drosophila cornichon gene product. J. Cell Biol. 142, 1209-1222.
VanRheenen, S.M., Cao, X., Lupashin, V.V., Barlowe, C. and Waters, M.G. (1998) Sec35p, a novel peripheral membrane protein, is required for ER to Golgi vesicle docking. J. Cell Biol. 141, 1107-1119.
Cao, X., Ballew, N. and Barlowe, C. (1998) Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins. EMBO J. 17, 2156-2165.
Barlowe, C. (1997) Coupled ER to Golgi transport reconstituted with purified cytosolic proteins. J. Cell Biol. 139, 1097-1108.
Belden, W. J. and Barlowe, C. (1996) Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient ER to Golgi transport. J. Biol. Chem. 271, 26939-26946.
Yeung, T., Barlowe, C. and Schekman, R. (1995) Uncoupled packaging of targeting and cargo molecules during transport vesicle budding from the endoplasmic reticulum. J. Biol. Chem. 270, 30567-30572.
Schimmoller, F., Singer-Kruger, B., Schroder, S., Kruger, U., Barlowe, C. and Riezman, H. (1995) The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi. EMBO J. 14, 1329-1339.
Barlowe, C., Orci, L., Yeung, T., Hosobuchi, D., Hamamoto, S., Salama, N., Rexach, M., Ravazzola, M., Amherdt, M. and Schekman, R. (1994) COPII: A membrane coat formed by Sec proteins that drive vesicle budding from the ER. Cell 77, 895-907.
Invited Reviews:
Barlowe, C. (2003) Molecular recognition of cargo by the COPII complex: a most accommodating coat. Preview article, Cell 114, 395-397.
Barlowe, C. (2003) Signals for export from the ER: what's the ticket out? Trends in Cell Biology 13, 295-300.
Barlowe, C. (2002) Three-dimensional structure of a COPII prebudding complex. Preview article, Developmental Cell 3, 467-468.
Barlowe, C. (2002) COPII-dependent transport from the endoplasmic reticulum. Curr. Opin. Cell Biol. 14, 417-422.
Barlowe, C. (2000) Traffic COPs of the Early Secretory Pathway. Traffic 1, 371-377.